Apps/Library prep
Library prep app · Roche chemistry (RUO)

Roche's KAPA EvoPlus Boost Kit (RUO), automated on Callisto™

Volta's app on Callisto for Roche's KAPA EvoPlus Boost Kit (RUO) converts DNA into Illumina-compatible, sequencing-ready libraries in a single automated process.

Illumina-compatible
Roche chemistry (RUO)
Output
Sequencing-ready libraries
Hands-on time
Less than 30 minutes
Batch size
4 to 24 reactions per run
Chemistry
Roche KAPA EvoPlus Boost (PCR-free) Kit (RUO)

Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.

Callisto Sample Prep System, closed front view, which runs the KAPA EvoPlus Boost App
Workflow

How it runs on Callisto

01

Load

Load DNA with the Callisto Kit for KAPA EvoPlus Boost and KAPA reagents.

02

Fragment and A-tail

Combined enzymatic fragmentation and A-tailing ReadyMix chemistry runs on deck.

03

Ligate and size-select

Adapter ligation and bead-based size selection, walk-away.

04

Libraries out

Illumina-compatible, sequencing-ready libraries.

✓
No new instrument

Run a new app on the Callisto™ you already have.

✓
No re-automation project

The protocol is already validated and automated.

✓
No new method development

Load the kit and walk away.

FAQ

Frequently asked questions

What does the KAPA EvoPlus Boost App on Callisto do?

It automates Roche's KAPA EvoPlus Boost Kit (RUO) workflow, converting DNA into Illumina-compatible, sequencing-ready libraries.

Which steps are automated?

Enzymatic fragmentation and A-tailing, adapter ligation and bead-based size selection, in a single automated process.

How much hands-on time does it take?

Less than 30 minutes, with 4 to 24 reactions in a single run.

What applications does the app support?

Whole-genome sequencing, whole-exome sequencing and targeted sequencing.

See KAPA EvoPlus Boost Library Prep on a Callisto in your lab.

Book a demo, or browse the full Callisto app menu.

Callisto™ is For Research Use Only. Not for use in diagnostic procedures. Oxford Nanopore products are RUO. Regulatory status and claims regarding third-party products are the responsibility of the third party.