High-quality WGS starts with better sample prep.

Callisto™ delivers fully automated, pre-validated workflows for whole genome sequencing—so your lab can scale with confidence, consistency, and the quality standards needed for clinical use.

Say goodbye to tedious clinical WGS prep and unpredictable outcomes.

Why choose Callisto:

With Callisto, every run delivers the same high-quality libraries—regardless of operator skill level—thanks to pre-validated workflows, precision liquid handling, and tightly controlled processing conditions. This unmatched consistency means fewer repeats, cleaner data, and greater confidence in every result.

WHY CALLISTO FOR WGS

Consistently Superior Results

Callisto delivers  highly uniform fragment sizes, and cleaner libraries for every batch. Designed to meet the rigid standards of clinical‑grade sequencing.

Fast Setup, Minimal Oversight

Achieve full setup in just 30 minutes, with 3 hours of hands-free runtime—allowing your team to focus on higher-value tasks without sacrificing throughput.

Turnkey and Ready on Day One

Pre-loaded and pre-validated WGS workflows let you start sample preparation immediately—no method development, optimization, or costly trial-and-error. Callisto gets you from first run to production faster, accelerating your path to high-quality data and results. Focus on discoveries, not delays.

Effortless Operation with No Expertise Needed

A clean, intuitive interface and guided workflow mean even users with minimal lab experience can run complex WGS preparations. Push-button simplicity—load your samples, select your app, and walk away.

BROAD NGS COMPATIBILITY

Callisto also offers broad NGS compatibility for both long- and short-read sequencing—making it ideal for expanding your test menu without costly new equipment and method development investments.

Built to Flex with Your Platform

Callisto works across all major sequencing platforms—so you don’t have to lock your prep into one pipeline.

DNA Library Prep for Illumina

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DNA Library Prep for PacBio SMRTbell™ 3.0

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DNA Library Prep for Oxford Nanopore, LSK114 (NEW*)

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TRUSTED BY LEADERS 
IN GENOMICS

Hartwig Medical Foundation: Co-developing applications for cfDNA, tissue, and long-read sequencing workflows

Callisto already delivers an excellent hybrid capture workflow… we are now working together to enable whole genome sequencing on various DNA sources…”

Ewart de Bruijn

Hartwig Medical Foundation

UMC Utrecht: Integrating Callisto into routine clinical WGS workflows

Callisto's walk-away automation, powered by advanced digital fluidics, is truly a game-changer. It allows our clinical technicians to simply load 24 DNA samples and produce clinical-grade sequencing-ready libraries seamlessly in <3 hours.”

Marcel Nelen

UMC Utrecht

CALLISTO VS. 
TRADITIONAL PREP

(COMPARISON TABLE)
Callisto
Manual
Liquid Handler
Hands-on time
<30 min
3–5 hrs
2–3 hrs
Operator walk away time
True uninterrupted walk-away automation – load and go
None – requires constant hands-on work
Limited walk-away capability – requires frequent operator intervention
Throughput (libraries/day)
72 Illumina
48 PacBio
72 Oxford Nanopore
8–12
24-96
Cross-platform sequencing compatibility
Fully compatible, no reprogramming needed
Protocol specific training required
Limited compatibility, requires reprogramming

RESOURCES

Digital Fluidics Simplifies NGS Library Preparation in a Clinical Setting

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Digital Fluidics Simplifies NGS Library Preparation in a Clinical Setting

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Digital Fluidics Simplifies NGS Library Preparation in a Clinical Setting

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UMC Utrecht adopts Volta Labs Callisto Sample Prep System to process thousands of samples for Whole Genome Sequencing-based prenatal and postnatal testing

Bridging the Gap in High Molecular Weight DNA Extraction

Assessment of a Novel Digital Fluidics Platform for Rapid Sample Processing from Whole Blood to Library

Digital Fluidics Simplifies NGS Library Preparation in a Clinical Setting

Automated Hybridization Enrichment of Hartwig OncoAct Panel Using Volta Labs Digital Fluidics Platform

READY TO MAKE CLINICAL WHOLE GENOME SEQUENCING LIBRARY PREP EASIER?

WEBINAR
WGS Without Limits:
Powering Clinical Genetics at UMC Utrecht with Callisto
DATE: Tuesday, 7 October 2025
TIME: 7AM PT | 10AM ET | 4PM CEST
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Bert van der Zwaag
Clinical Genetic Laboratory, UMC Utrecht