twist fast hybridization capture
SAME-DAY ENRICHMENT, WITHOUT COMPROMISE
The Twist Fast Hyb App on Callisto delivers high-performance target enrichment using Twist Bioscience's trusted chemistry—automating the workflow from library load through to libraries ready for amplification.
key benefits
- Faster Turnaround: Enriched, sequencer-ready libraries in 6 hours.
- Uncompromised Performance: The uniform coverage and on-target specificity you expect from Twist chemistry—now at Fast Hyb speed.
- Simpler workflow: Streamlined capture anyone in the lab can run, with consistent, operator-independent results.
The Twist Fast Hybridization Capture workflow delivers highly enriched libraries in as little as 6 hours—compressing a traditionally overnight, multi-step protocol into a single day.After a brief bench setup of the hybridization reactions, Callisto automates the rest: the two-to-four-hour hybridization, target capture, heated washes, and PCR setup. Libraries come off Callisto only for PCR amplification, then optionally return for automated post-PCR cleanup.
App Specifications
Samples & Throughput
Input:
500 ng - 1.5 μg DNA libraries
500 ng - 1.5 μg DNA libraries
Multiplexing:
Flexible, 1 - 8 libraries
Flexible, 1 - 8 libraries
Throughput:
4 reactions/run
4 reactions/run
Chemistry & Output
Panel: Twist exome and custom hybridization panels
Chemistry:
Twist Fast Hyb Kit
Twist Fast Hyb Kit
Output:
Enriched libraries ready for PCR
Enriched libraries ready for PCR
Speed
Hands on Time:
<30 mins
<30 mins
Total Workflow Time:
6-8 hours
6-8 hours
Uniformity and on-target rates match manual Twist workflows
Callisto Fast Hyb App delivers on-target rate, uniformity (Fold-80), 30× target coverage, and zero-coverage targets comparable to manual Twist Standard Hyb v2 and Fast Hyb workflows. Data shown for the Twist Exome 2.0 panel, downsampled to 40 million reads.
Same-day enrichment with under 30 minutes hands-on
Callisto compresses a long, complex manual protocol into a streamlined, walk-away workflow—taking enriched libraries from load to sequencer-ready in a single day. Its ease of use also speeds adoption, minimizing the training needed to bring hybridization capture into your lab.
Highly reproducible run to run performance, with consistent on-target rates
Across three independent Callisto runs (12 libraries each), on-target rates remained tightly clustered with a coeffi cient of variation of 2%, confi rming consistent enrichment performance run to run. Data shown for the Twist Exome 2.0 panel.
To process samples using Twist Fast Hybridization Capture you will need the following materials:
Volta Labs
Callisto Kit for Twist Fast Hybridization Capture - 12 rxns
HC0003
Twist Bioscience*
Twist Fast Hyb and Wash Kit with TrueAmp Mix, 12 Reactions
116616
Twist Universal Blockers, 12 rxn
100578
Twist Binding and Purification Beads, 12 rxn
100983
Twist Hybridization Capture Panels
N/A
*Please contact Twist Bioscience directly to purchase these reagents.
Simply contact sales@twistbioscience.com for more information, or learn more at twistbioscience.com/products/ngs
Simply contact sales@twistbioscience.com for more information, or learn more at twistbioscience.com/products/ngs
To order: sales@voltalabs.co
