DNA Library Prep
for Oxford nanopore LSK114
Miniaturized Library Preps with High Yields and Sequencing Data
Volta’s DNA Library Prep for ONT (LSK114) App offers an automated, streamlined process to convert high molecular weight DNA into Oxford Nanopore-compatible, sequencing-ready libraries at a miniaturized reaction scale. This App supports whole-genome sequencing, targeted sequencing, and metagenomics applications.
key benefits
- Maximized output: Achieve >90 Gb on PromethION from a single library load over 72 hours.
- Lower input requirements: 2x higher library yield enables the same output with less DNA input.
- Reduced costs & time: Cut prep costs via 2x miniaturization and up to 6x less hands-on time.
The DNA Library Prep for ONT (LSK114) App offers robust library prep for various DNA sizes and sources. Callisto supports flexible batch sizes up to 24 reactions with a preloaded workflow to enable sequencing on Oxford Nanopore sequencing platforms. With a fully automated library prep, you spend less than 30 minutes of set-up time and achieve 3.5 hours of walk-away time for every 24 sample run on Callisto.
App Specifications
Input: 1 μg, or 100-200 fmol of high molecular weight DNA
Output: Sequencing ready libraries
Hands-on time: Less than 30 minutes
Walk-away time: 3.5 hours
Throughput: Flexible, 4 - 24 rxns in a single run
Chemistry: Ligation Sequencing Kit V14 + NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing
Miniaturized LSK114 preps with high yields
Callisto generates significantly higher library yields across a wide range of DNA input sizes compared to manual preps. 1 µg of input DNA yields enough library to load a PromethION flowcell and generate high quality sequencing data
Libraries retain integrity of input DNA
The App uses a gentle process that prevents shearing, preserving the integrity of high molecular weight input DNA in the output libraries and enabling high N50 values when sequenced. As shown in the figure, the size distribution of input DNA and output libraries demonstrates strong overlap with only minimal shift.
High quality sequencing on Oxford Nanopore sequencing platforms
Libraries prepared on Callisto deliver high-quality sequencing results on MinION and PromethION platforms with exceptional scores on key metrics including N50, Q score, and pore occupancy with single loading. Consistently generating >90 GB of data is now feasible on PromethION flowcell due to the high efficiency library preparation process. The figure shows data obtained on a PromethION flowcell that was loaded once with 400 ng of the libraries prepared on Callisto.
To process samples using DNA Library Prep for Oxford nanopore LSK114 you will need the following materials:
Volta Labs
DNA Library Prep for ONT (LSK114) - 48 rxns, Accessory Kit
LP0007
DNA Library Prep for ONT (LSK114) - 480 rxns, HT Accessory Kit
LP0010
ONT*
Ligation Sequencing Kit V14
SQK-LSK114
SQK-LSK114-XL
NEB*
NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing
E7672S
E7672L
*Please contact suppliers directly to purchase these reagents.
To order: sales@voltalabs.co
