VoltaPrep Logo

DNA Library Prep
for Oxford nanopore LSK114

Overview

Workflow and Specs

Data

Ordering

Resources

Simplify workflow with walk-away Oxford Nanopore library prep

Volta’s DNA Library Prep for ONT (LSK114) App offers an automated, streamlined process to convert high molecular weight DNA into ONT compatible sequencing-ready libraries, at a miniaturized reaction scale. The App supports whole-genome sequencing, targeted sequencing, and metagenomics applications.

key benefits

  • Prep 2x more reactions from library prep kit
  • Achieve highest quality libraries with sustained high pore occupancy
  • Get 3 hours of walk-away time for 24 sample library prep

ACCESS THIS APP ON CALLISTO

SCHEDULE A DEMO

The DNA Library Prep for ONT (LSK114) App offers robust library prep for various DNA sizes and sources. Callisto supports flexible batch sizes up to 24 reactions with a preloaded workflow to enable sequencing on Oxford Nanopore sequencing platforms. With a fully automated library prep, you spend less than 30 minutes of set-up time and achieve 3 hours of walk-away time for every 24 sample run on Callisto.

A flowchart shows the process of preparing ONT libraries. The first step, represented by five black circles, is followed by a black arrow pointing to "DNA repair & end repair." A blue line beneath this box, extending to the right, indicates "WALK-AWAY PROCESSING ON CALLISTO." This blue line continues under "Clean up," "Adapter Ligation," and another "Clean up" step. All these steps are connected by black arrows. The final step, "OUTPUT: ONT libraries," is also connected by a black arrow and points to a single black circle.A vertical flowchart illustrates the process of preparing ONT libraries. The top box, labeled "INPUT: Sheared, unpurified DNA," contains five small white circles. Below it, a series of darker gray and black rectangular boxes are stacked, each representing a step in the process. From top to bottom, these steps are: "DNA repairs & end repair," "Clean up," "Adapter ligation," and another "Clean up." A blue horizontal line runs beneath the text in each of these four boxes. To the left of these four boxes, a vertical blue text label reads "WALK-AWAY PROCESSING ON CALLISTO." The final box at the bottom is labeled "OUTPUT: ONT libraries" and is followed by a single black circle.

App Specifications

Input: 1 µg of DNA
Output: Sequencing ready libraries
Hands-on time: Less than 30 minutes
Walk-away time: 3 hours
Throughput: Flexible, 4 to 24 rxns in a single run
Chemistry: Ligation sequencing DNA V14 (SQK-LSK114)

ACCESS THIS APP ON CALLISTO

SCHEDULE A DEMO

Miniaturized LSK114 preps with high yields

LSK114 library prep using 1 µg of high molecular weight DNA and half scale reagents generates ~700 ng of sequencing ready libraries. The App maintains the integrity of the high molecular weight input DNA in the output libraries.
The image contains two plots comparing miniaturized LSK114 preps. The left plot is a box plot showing "Library recovery (%)" on the y-axis for "Callisto" on the x-axis, indicating a median recovery around 70-75% with several data points scattered above and below the box. The right plot is a size distribution curve, with "Intensity" on the y-axis and "Size (kbp)" on the x-axis, displaying two overlapping curves: one in gray labeled "Post-shearing" and another in blue labeled "ONT Libraries," both peaking around 20 kbp, suggesting similar size distributions.

High quality sequencing on Oxford Nanopore sequencing platforms

Callisto-prepped libraries deliver high-quality sequencing results on MinION with exceptional scores on key metrics including N50, Q score, and pore occupancy. For this data set, the flowcell was loaded with 300 ng of the Callisto prepared library.
The image displays three plots related to sequencing data analysis. The top plot is a histogram showing "Bases (Mb)" on the y-axis against "Read Length (kb)" on the x-axis, with a peak around 10-20 kb, and a text label "N50: 15.12 kb." The middle plot is another histogram, depicting "Bases" on the y-axis against "Q Score" on the x-axis, with the highest bars appearing between Q scores of 20 and 30. The bottom plot is a scatter plot showing "Pore Occupancy (%)" on the y-axis against "Sequencing Time (h)" on the x-axis, with a high concentration of points near 80% pore occupancy throughout the sequencing duration.

ACCESS THIS APP ON CALLISTO

SCHEDULE A DEMO
To process samples using DNA Library Prep for Oxford nanopore LSK114 you will need the following materials:
Volta Labs
DNA Library Prep for ONT (LSK114) - 48 rxns Accessory Kit
LP0007
ONT*
 Ligation Sequencing Kit V14
SQK-LSK114
SQK-LSK114-XL
NEB*
NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing
E7672S
E7672L
*Please contact our partners directly to purchase these reagents.
To order: sales@voltalabs.co

ACCESS THIS APP ON CALLISTO

SCHEDULE A DEMO
Data sheet
DNA Library Prep for Oxford nanopore LSK114 (COMING SOON!)

ACCESS THIS APP ON CALLISTO

SCHEDULE A DEMO