HYBRIDIZATION CAPTURE FOR TWIST (STANDARD V2)
BEST-IN-CLASS ENRICHMENT, SIMPLIFIED FOR EVERY LAB
The Hybridization Capture for Twist (Standard v2) App on Callisto delivers high-performance hybridization capture using Twist Bioscience's trusted chemistry—fully automated from input libraries to enriched, PCR ready libraries.
Harness the power of Twist’s hybrid capture chemistry with Callisto's walk-away platform for unmatched performance and simplicity.
key benefits
- Consistent Performance: Callisto handles hybridization, heated washes with reproducible enrichment results.
- Uniform Coverage: Ensure balanced enrichment across all GC regions.
- Simpler workflow: A simpler capture workflow that anyone in the lab can run.
The Twist Hybrid Capture workflow delivers highly enriched libraries in just 24 hours.
Users begin by setting up hybridization reactions at the bench, after which Callisto runs the overnight hybridization step. The following day, capture beads and wash buffers are loaded onto Callisto, which automates target capture, heated washes, and PCR setup. The enriched libraries are then taken off Callisto for PCR amplification, and the amplified libraries can optionally be returned to Callisto for automated post-PCR cleanup.
App Specifications
High quality sequencing metrics
Uniform coverage across targets
Excellent target coverage for difficult to capture regions (ACTA1and RASA4B exon)
The partnership between Twist Bioscience and Volta combines Twist’s industry-leading hybrid capture chemistry with Volta’s fully automated Callisto platform. Together, this collaboration enables researchers and clinical laboratories to achieve highly consistent enrichment performance through a streamlined, simplified workflow.