Watch this webinar on demand: Volta + IDT Precision at Scale - Revolutionizing Genomic Sample Preparation
On January 14, 2025, Volta Labs and IDT partnered to produce this webinar presented by Abdul Mohammed, Head of Applications Development at Volta Labs, and Nick Downey, NGS Collaborations Lead at IDT. They discuss performance data from Volta's IDT xGen™ NGS Hybridization Capture app on the Callisto Sample Prep System and how Callisto addresses existing challenges to offer an innovative and reliable solution for automating hybridization capture workflows.
To evaluate Volta's IDT xGen™ NGS Hyb Capture app performance on Callisto, three experiments were performed in this study. Phase 1 and 2 evaluate inter-user variability of single-plex libraries of the Exome v2 Hyb panel and the Pan-Cancer Hyb panel, respectively, compared directly to manually prepared libraries. Phase 3 evaluates multiplexing on Callisto, from 1-plex all the way up to 20-plex libraries.
Phase 1: Operator variability with large panels using the xGen IDT Exome V2 panel, comparing directly against manual preparations
- 100 ng HG002 prepared using the xGen™ DNA Lib Prep EZ for exome, xGen Exome Hyb Panel v2
- This panel has approximately 415K individual probes spanning 34 Mb of target region for the human exome
- Sequenced on the NextSeq 2000 (2X150 cycles), using P4 XLEAP chemistry
- Downsampled to 50M PE reads total across all samples
Phase 2: Operator variability with small panels using the xGen Pan-Cancer, comparing directly against manually preparations
- 20 ng Horizon’s OncoSpan cfDNA prepared using the xGen™ cfDNA & FFPE DNA Library Prep v2 MC Kit, xGen Pan-Cancer Hyb Panel
- This panel has approximately 7800 probes spanning 800 kb of target region for onco-genes
- Sequenced on the NextSeq 2000 (2X150 cycles), using P1 XLEAP chemistry
- Downsampled to 4M PE reads total across all samples
Phase 3: Performance of high-plexity libraries on Callisto
- 100 ng HG002 prepared using the xGen™ DNA Lib Prep EZ for exome, xGen Exome Hyb Panel v2
- 4 captures were performed: 1X, 8X, 12X, 20X
- With higher plexity libraries with 20 pooled samples, you have a much lower ratio of probe:sample
- We are assessing the limits of performance with this kit with various plexities, from 1-plex all the way up to 20-plex
- With 20-plex, some of the samples are extremely viscous, with 10 µg of DNA in the pool
Key takeaways:
- Walk-away, automated solution for complex end-to-end hybridization capture workflows
- High quality, high-plexity enrichment performance independent of operator
- Consistent coverage uniformity and high on-target bases for different panel sizes
- Callisto reduces hands on time, saving operators 2.5 hours of hands-on time
- Flexible throughput to match lab needs
- Minimal training required with less than 30 minutes of set-up time per run
Contact our team to discuss how you can automate hybridization capture with the Callisto Sample Prep System.
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Introducing VoltaHyb:
Automating the Last Mile of Targeted Sequencing Workflows
The Volta Labs team is revolutionizing critical targeted sequencing workflows with the introduction of the VoltaHyb™ suite of Apps and kits for the Callisto™ Sample Prep System. Designed to enrich your targets with confidence every single time, the Callisto platform brings unmatched workflow simplicity to hybridization capture. With Callisto, users no longer need to rely on multiple lab instruments (heated blocks, magnetic racks, vortexers) or perform time-sensitive heated transfer steps - such complex steps are abstracted away from the end user. Furthermore, Callisto's unique architecture enables precise magnetic bead manipulation coupled with uninterrupted and uniform temperature control during the hybrid capture enabling consistent enrichment performance.
Callisto reduces sequencing redo rates to less than 3% while minimizing sample loss throughout the capture process. This breakthrough approach enables reliable targeted sequencing even for challenging applications where conventional tools struggle. These advantages extend across both long and short read-based hybrid capture workflows.
The scalable architecture of the Callisto system enables flexible throughput, with the capacity to process up to 96,000 exomes per year or 48,000 PacBio HiFi samples annually. Whether you're running a small research lab or a high-volume testing facility, Callisto adapts to your needs with flexible batch sizes and consistent performance.
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Key Benefits
of VoltaHyb for Hybridization Capture:
Reduced Sequencing Costs:
Lower sample preparation and sequencing costs by minimizing reruns (<3% redo rates).Fully Automated Workflow:
Enables complete walk-away automation for hybridization, capture, washes, and PCR setupBroad Panel Compatibility:
Works with panels of all sizes, from small targeted panels to comprehensive exomesSupport for Both Short and Long Read Applications:
Optimized protocols for both Illumina-style short-read sequencing and long-read technologies like PacBio and Oxford Nanopore
Consistent Results:
Dramatically reduced inter-operator variability and robust performance across repeated runsUniform Coverage:
Even coverage across challenging regions, including high-GC content areasMaximum Throughput: Process up to 480 exomes simultaneously with pooled libraries with flexible batching
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VoltaHyb’s Expanding App Menu
At Volta, we’re continually growing our support for hybrid capture workflows across both short- and long-read sequencing platforms.
On the short-read side, Callisto currently supports IDT xGEN v2 chemistry, as well as Twist’s standard v2 and Fast chemistries. For long-read applications, we support PacBio HiFi enrichment based on Twist’s standard v2 chemistry.
There are more Apps coming to the VoltaHyb menu, and we’re always listening to our users. If there’s a hybrid capture chemistry you’d like to see automated on Callisto, let us know—we’d love to hear from you.
for Twist, v2 standard
The revolution in genomic sample preparation is here. By bringing the push-button experience of a sequencer to targeted sequencing workflows, Volta Labs is transforming how laboratories approach hybridization capture. The choice is no longer between inconsistent manual methods and complex automation platforms that require dedicated engineering resources. With Callisto and VoltaHyb, you can achieve consistent, high-quality results without specialized training or extensive method development.
Ready to experience the difference? Contact our team today to learn how the VoltaHyb powered Hybridization Capture can enhance your targeted sequencing workflows and accelerate your discoveries.
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Automating the Last Mile of Targeted Sequencing Workflows on Callisto
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